Transcriptionally active regions for transgene integration
Sofie O’Brien et al. combined information on transcriptional activity from RNAseq data and chromatin accessibility from ATAC sequencing to identify desirable sites for transgene integration. This approach streamlines the development of new product producing cell lines.
(Biotechnol. J. 13: 1800226. DOI 10.1002/biot.201800226)
Meta-analysis of transcriptomes for hepatocyte cell differentiation
Ravali Raju et al. described a meta-analysis approach that enabled gene expression data of in vitrodifferentiation of hepatocyte-like cells from stem cells and mouse embryonic liver development to be integrated for analysis. Through aligning the metadata chronologically they concluded that the current HLC derived from pluripotent stem cells all reached an immature stage that is equivalent to mouse embryo liver at ~E14.
(Stem Cells and Development, 27:910-921, DOI 10.1089/scd.2017.0270)