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Fundamentals and Frontiers This intensive short course is for engineers, cell biologists, and scientists interested in state-of-the-art cell culture technology. This course has thus far attracted participants from more than 35 countries since it was first offered in 1986. This course focuses on the fundamentals of cell biology and biochemical engineering. A new format for lectures and notes has been adopted to emphasize the major strength of this course: the essential information and analysis is packaged and delivered in a most comprehensible fashion to the audience by experts in the field. After a concise review of the current issues in cell biology and physiology, course participants are introduced to the rationale behind the selection, operation and design of cell culture reactors and to overall process design and optimization. Included in this offering is an in-depth look at various analytical techniques for process characterization, cell culture technology in tissue engineering and gene therapy. Emphasis is given to new instrumentation developments and strategies for the control of nutrient feeding and perfusion for both cell culture and tissue engineering. New processing technology and future prospects of vaccines and other biologicals are also presented. Audience To benefit most from this course, participants should have either a general understanding of bioprocessing or a familiarity with microbial or cell culture and tissue processes. For bioprocessing scientists and engineers, this course is an up-to-date intensive review and analysis of cell culture processes. For those participants new to cell culture, the course is an important overview to cell technology and an efficient introduction to critical issues in cell culture process development. For experienced cell culture technologists, this course offers a refreshing look and critical evaluation of various aspects of the technology. Format Topics are presented through lectures accompanied by an extensive set of course notes and a CD-Rom. The course notes include relevant data from the literature, concise summaries and analyses. This valuable resource of information has been packaged to facilitate understanding and for quick future referencing. Extensive problem solving and examples are used to illustrate the application of engineering principles to kinetic analysis and to cell and tissue reactor and process design. Course lectures are supplemented with discussion sessions, a tutorial computer session on data analysis and simulation, and, for the Minneapolis course, laboratory demonstrations and poster sessions. For further information,
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