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Events
Progress and Business Meeting for Consortium Members
May 1, 2007
Minneapolis, MN
The simi-annual meeting for consortium members was held at the University of Minnesota in May 2007. At the meeting, members were updated on the progress made since the training session in October 2006. Testing and validation of the first generation Affymetrix microarray, current sequencing efforts, proteomics work, and improved annotation strategies were the highlights of the progress made in the past six months. Discussion of continued sequencing strategies, microarray development, and new research directions were also addressed by collaborators and participants. Plans for the next annual meeting and data analysis workshop this fall were also discussed.
CHO Consortium Welcomes Katie Wlaschin
March 2007
Katie Wlaschin has joined the Consortium for CHO Cell Line Genomics as a technical specialist. Her work in collaboration with the Bioprocessing Institute of Singapore provided much of the foundation for the formation of the Consortium. Her experiences and expertise will be an asset in directing future work and providing technical assistance to consortium members.
Training Workshop on Microarray Data Analysis
October 12-13, 2006
A workshop was held to provide cell culture professionals in member companies with the technical knowledge and tools required to fully utilize the power of transcriptome and proteome analysis. The workshop focused on the importance of using a synergetic approach including statistical analysis, bioinformatics, and knowledge of cellular physiology to extract the most information from DNA microarray and proteomic data. A hands-on workshop covered the fundamentals of microarray data analysis, with references to ways to assimilate analysis of proteomic data. The use of software and data mining to assist in development of physiological insight from transcriptome data was emphasized.
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Workshop Lecture Topics:
Overview of Transcriptome Analysis
Application of Genomic Tools in Mammalian Cell Culture
Experimental Protocols
Microarray Experimental Design
Data Processing and Normalization
Differential Expression Analysis
Time Series Data Analysis
Pathway and Network Analysis
Pattern Recognition
Mining Microarray Data
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Milestones
| July 2001 |
Construction of first CHO EST Library |
| June 2002 |
Construction of second CHO EST Library |
| January 2003 |
Printing of first CHO cDNA microarray (4,300 sequences). |
| January 2003 |
Continued EST sequencing of EST libraries to > 16,000 transcripts |
| May 2003 |
Presentation of "Genomic Exploration in Chinese Hamster Ovary Cells" 18th Annual ESACT Meeting, Spain |
| December 2003 |
Printing of second CHO cDNA microarray (16,000 sequences) |
| March 2004 |
Presentation of "Gene Discovery in CHO Cells by EST Sequencing, Microarray and a Comparative Sequence Database" , Cell Culture Engineering IX, March 7-12, 2004, Cancun, Mexico |
| July 2005 |
First meeting to explore the formation of the CHO Consortium |
| October 2005 |
Construction of third generation CHO and hamster EST libraries |
| February 2006 |
Inaugural meeting of the Consortium for Chinese Hamster Ovary (CHO) Cells Genomic Research |
| December 2006 |
First generation of Affymatrix DNA array |
| October 2006 |
Consortium Training Workshop on Microarray Data Analysis |
| December 2006 |
First generation CHO Affymetrix Microarray |
| January 2007 |
Construction and sequencing of fourth generation CHO EST libraries |
| May 1, 2007 |
Consortium members meeting, Minneapolis, MN |
| On-going |
Development of second generation CHO Affymetrix Microarray |
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