| Biochemical Engineering X | May 18-23, 1997 | Kananaskis, Alberta, Canada |
| Conference | Wei-Shou Hu, University of Minnesota |
| James R. Swartz, Genentech |
| May 18, Sunday |
| 2:00 - 5:30 p.m. | Registration |
| 6 :00 p.m. | Dinner |
| Wei-Shou Hu and James R. Swartz |
| 7:40 p.m. | Arthur E. Humphrey | Penn State University | 1-1 |
| Shake flask to fermentor, what have we learned? |
| 8:10 p.m. | Daniel I.C. Wang | Massachusetts Institute of Technology | 1-2 |
| The role of biochemical engineering in new biotechnology |
| 8:40 p.m. | James E. Bailey | ETH | 1-3 |
| Mathematical modeling and analysis in biochemical engineering: Past |
| accomplishments and future opportunities |
| 9:30 - 11:00 p.m. | Reception |
| May 19, Monday |
| 7 :00 a.m. | Breakfast |
| 8:30 a.m. - 12:30 | Cellular and Molecular Technology |
| George Georgiou |
| 8 :30 a.m. | George Georgiou | University of Texas at Austin |
| Introduction |
| 8:35 a.m. | Jay D. Keasling | University of California-Berkeley | 2-1 |
| Development of tools for the metabolic engineering of bacteria |
| 9:05 a.m. | Sang Yup Lee | KAIST | 2-2 |
| Metabolic engineering and fermentation of polyhydroxyalkanoate |
| 9:35 a.m. | Douglas C. Cameron | University of Wisconsin | 2-3 |
| Design, construction and analysis of metabolic pathways for the |
| production of diols |
| 10:05 | Coffee Break |
| 10:30 | George Georgiou | University of Texas at Austin | 2-4 |
| Molecular engineering of antibodies |
| 11:00 | K. Dane Wittrup | University of Illinois | 2-5 |
| Antibody engineering in yeast |
| 11:30 | Larry V. McIntire | Rice University | 2-6 |
| Differential gene regulation induced by flow in vascular cells: |
| Implications for gene therapy |
| 12:00 | Vasantha Nagarajan | E I. du Pont de Nemours, Inc. | 2-7 |
| Identification of key parameters to model metabolic capabilities of |
| wastewater bioreactors |
| 12:30 p.m. | Lunch |
| 5 :00 - 6:00 p.m. | Poster Session I and Social Hour |
| Karen McDonald and Florence Wu |
| Poster Session I authors will present posters |
| 6 :00 p.m. | Dinner |
| 7:30 - 9:40 p.m. | Understanding Bioprocess Unit Operations |
| Murray Moo-Young and Alvin Nienow |
| 7 :30 p.m. | Murray Moo-Young | University of Waterloo |
| Introduction |
| 7:35 p.m. | Alvin Nienow | University of Birmingham | 3-1 |
| Status Review of Mixing in Bioreactors |
| 8:05 p.m. | Harvey W. Blanch | University of California | 3-2 |
| Protein interactions in solution: Applications of molecular |
| thermodynamics to protein precipitation and crystallization |
| 8:45 p.m. | Michael L. Shuler | Cornell University | 3-3 |
| Developing a rational basis for scale-up of microcarrier cultures: |
| Relating "local" hydrodynamics to cellular physiology |
| 9:05 p.m. | Konstantin Konstantinov | Bayer Inc. | 3-4 |
| Optimization of high-density perfusion cultures based on criteria for |
| gross dollar profit |
| 9:20 p.m. | Murray Moo-Young | University of Waterloo | 3-5 |
| Developments in bioprocess intensification technology |
| 9 :40 - 10:30 p.m. | Poster Session II and Social Hour |
| Karen McDonald and Florence Wu |
| Poster Session II authors will present posters |
| May 20, Tuesday |
| 7:00 a.m. | Breakfast |
| 8:30 a.m. - 12:15 | Bioprocess Information |
| Suteaki Shioya and Thomas Scheper |
| 8 :30 a.m. | Suteaki Shioya | Osaka University |
| Introduction |
| 8:35 a.m. | Thomas H. Scheper | Institut fur Tech. Chemie | 4-1 |
| Optical sensor systems for the on-line monitoring of bioprocesses |
| 9:05 a.m. | Nazmul Karim | Colorado State University | 4-2 |
| Status Review of Application of Neural network/PCA in bioprocessing |
| 9:35 a.m. | Michael Hoare | University College London | 4-3 |
| Enhanced bioprocess piloting by means of scale-down and rapid |
| monitoring of product and key contaminants |
| 10:05 | Coffee Break |
| 10:30 | Isao Endo | RIKEN, The Institute of Physic | 4-4 |
| A man-machine interface for maintenance of bioplant---scheduling of |
| maintenance by multiple robots |
| 11:00 | Pradyumna Ku Namdev | Schering-Plough Research Institute | 4-5 |
| Sniffing out trouble: Use of electronic nose in fermentation |
| 11:30 | Suteaki Shioya | Osaka University | 4-6 |
| On-line fault diagnosis for an optimal rice alpha-amylase production |
| process of temperature-sensitive mutant of saccharomyces cerevisiae |
| by autoassociative neural network |
| 12:10 | Thomas H. Scheper | Institut fur Tech. Chemie |
| Concluding Remarks |
| 12:15 p.m. | Lunch |
| 4 :15 - 5 :15 p.m. | Workshop: Regulatory Issues |
| Yashwant Deo, Renato Fuchs and Anthony Lubiniecki |
| Kenneth Seamon | Immunex Corporation |
| Anthony Lubiniecki | SmithKline Beecham |
| Yashwant Deo | Medarex |
| Renato Fuchs | Chiron |
| 6 :00 p.m. | Dinner |
| 7:30 - 9:35 p.m. | Product Formulation Fundamentals |
| Jeffrey Cleland |
| 7:30 p.m. | Jeffrey L. Cleland | Genentech Inc. |
| Introduction |
| 7:35 p.m. | Ronald T. Borchardt | University of Kansas | 5-1 |
| Chemical instability of proteins |
| 8:05 p.m. | Steven L. Nail | Purdue University | 5-2 |
| Analysis of the dynamics of freeze drying |
| 8:35 p.m. | Jeffrey L. Cleland | Genentech Inc. | 5-3 |
| Solvent evaporation processes for the production of controlled release |
| therapeutics and vaccines |
| 9:05 p.m. | Mark Tracy | Alkernes, Inc. | 5-4 |
| Development and scale-up of a microsphere protein |
| 9 :35 - 10:30 p.m. | Poster Session I and Social hour |
| Karen McDonald and Florence Wu |
| Poster Session I authors will present posters |
| May 21, Wednesday |
| 7:00 a.m. | Breakfast |
| 8:30 a.m. - 12:00 n | Bioprocessing for Large Scale Production of Plant Based |
| Charles E. Wyman and Lee R. Lynd |
| 8:30 a.m. | Lee R. Lynd | Dartmouth College | 6-1 |
| Plant based bioprocessing overview & reactor and organism design for |
| processing cellulosic biomass |
| 9 :10 a.m. | Mark Worden | Michigan State University | 6-2 |
| Gas-liquid mass transfer in production of large-scale bioproducts with |
| application to synthesis gas processing |
| 9 :40 a.m. | Ponnapalam Elankovan | Michigan Biotechnology Institute | 6-3 |
| Separation of commodity bioproducts: Establishing the fundamental |
| and applied foundation |
| 10:10 | Coffee Break |
| 10:40 | Roger Timmis | Weyerhaeuser Company | 6-4 |
| Bioprocessing in the forest products industry |
| 11:10 | Donald L. Johnson | Grain Products Corporation | 6-5 |
| Bioprocessing in the agricultural products industry |
| 11:40 | Charles E. Wyman | 6-6 |
| Process economics overview and concluding remarks |
| 12:00 n | Lunch |
| 4 :00 - 5 :00 p.m. | Workshop: Innovations in Biochemical Engineering |
| James Liao, Sadettin Ozturk and Friedrich Srienc |
| James Liao | Texas A&M University |
| Sadettin Ozturk | Bayer |
| Friedrich Srienc | University of Minnesota |
| 5 :00 - 6 :00 p.m. | Workshop: Trends for the Future in Biotechnology |
| Konstantin Konstantinov and Rolf G. Werner |
| Rolf G. Werner | Dr. Karl Thomae GmbH |
| Safe and economic expression of therapeutic proteins |
| Konstantin Konstantinov | Bayer Inc. |
| Trends in fermentation technology |
| Juan A. Asenjo | Universidad de Chile |
| Expert systems in rational protein purification |
| Steve Lee | Merck & Co |
| Straight-through process technology for finishing steps |
| 6 :00 p.m. | Dinner |
| 7:30 - 9:10 p.m. | New Medical Applications |
| Mark W. Saltzman |
| 7:30 p.m. | David Mooney | University of Michigan | 7-1 |
| Synthetic extracellular matrices to recreate 3-D cell-cell interactions |
| 7:50 p.m. | Kevin E. Healy | Northwestern University | 7-2 |
| Biomolecular engineering of surfaces to manipulate cell function |
| 8:10 p.m. | J. H. David Wu | University of Rochester | 7-3 |
| Engineering a bone marrow mimicry multilineal hemopoiesis in a |
| three-dimensional bone marrow bioreactor |
| 8:30 p.m. | Mark W. Saltzman | Cornell University | 7-4 |
| Passive and active immunization using polymer materials |
| 8:50 p.m. | Jeff J. Chalmers | Ohio State University | 7-5 |
| Development of a continuous, magnetic cell separator |
| 9 :15 - 10:30 p.m. | Posters and Social Hour |
| May 22, Thursday |
| 7:00 a.m. | Breakfast |
| 8:30 - 11:50 a.m. | Biopharmaceuticals Production |
| Dane Zabriskie and Vijay M. Yabannavar |
| 8:30 a.m. | Dane Zabriskie | Smith-Kline Beecham |
| Introduction |
| 8:35 a.m. | Michael R Ladisch | Purdue University | 8-1 |
| Engineering analysis of transport phenomena in aligned fiber |
| (continuous stationary phase) chromatography columns |
| 9:05 a.m. | Steven M. Cramer | Rensselaer Polytechnic Institute | 8-2 |
| Protein purification from complex biological mixtures using high |
| throughput gradient and displacement chromatography |
| 9:35 a.m. | Matthew S. Croughan | Genentech Inc. | 8-3 |
| Prediction of culture performance through cell cycle analysis: a tool in |
| operations scheduling |
| 10:05 | Coffee Break |
| 10:30 | Eliana de Bernardez-Clark | Tufts University | 8-4 |
| In vitro protein folding and recovery of inclusion body proteins |
| 11:00 | Sadettin S. Ozturk | Bayer Corporation | 8-5 |
| Biochemical engineering approaches to industrial process development |
| for mammalian cell culture based biologicals |
| 11:30 | James R. Swartz | Genentech, Inc. | 8-6 |
| Genetic approaches to the detection of contaminants in e. coli |
| 11:50 | Vijay M. Yabannavar | Chiron |
| Conclusion |
| 12:00 n | Bus departs for Lake Louise |
| 1 :30 p.m. | Arrive at Lake Louise |
| 4 :30 p.m. | Bus returns from Lake Louise |
| 6 :00 p.m. | Arrive at Kananaskis |
| 7:00 p.m. | Conference Banquet and Amgen Award Lecture |
| Wei-Shou Hu and James Swartz |
| Michael L. Shuler | Cornell University |
| Amgen Award Lecture |
| 9 :30 p.m. | Social Hour |
| May 23, Friday |
| 7:00 a.m. | Breakfast |
| 8 :30 - 11:40 a.m. | Frontiers in Biochemical Engineering |
| Isao Endo and Douglas Clark |
| 8:30 a.m. | John A. Keller | SmithKline Beecham Pharmaceuticals | 9-1 |
| The scale-up of drug discovery |
| 9:10 a.m. | Alan J. Russell | University of Pittsburgh | 9-2 |
| Biotechnology versus chemical weapons: A battle for the 21st |
| 9:40 a.m. | Douglas S. Clark | University of California | 9-3 |
| Combinatorial biocatalysis for drug discovery and optimization |
| 10:10 | Coffee Break |
| 10:40 | Pauline M. Doran | University of New South Wales | 9-4 |
| Cross-species co-culture of plant organs for high level production of |
| secondary metabolites |
| 11:10 | Zivko L. Nikolov | Iowa State University | 9-5 |
| Production and purification of recombinant proteins from transgenic |
| 11:40 a.m. | Conference Closing |
| 12:00 n | Lunch |