Intensive Short Course at Novozymes - Adelaide, Australia
Prof. Hu traveled to Adelaide Australia to give an abbreviated version of the annual course offered at the University of Minnesota.
Cellular Engineering - A special short course in Dalian, China - October 12th This intensive short
course is an abbreviated version of the annual course offered at the University of Minnesota. The course is specifically designed for engineers, cell biologists, and scientists interested in
state-of-the-art cell culture technology. This course
has thus far attracted participants from more than 35 countries since
it was first offered in 1986. The course focuses on the fundamentals
of cell biology and biochemical engineering.
Training Workshop on Microarray Data Analysis for Cell Culture Engineering This hands-on workshop was held June 19-20, 2008. The workshop covered fundamentals of microarray data analysis with references to proteomic analysis. Additionally, lab members, Anne Kantardjieff, Nitya Jacob and Huong Le went to Singapore at BTI Singapore and gave the workshop to our collaborators there. During that trip they also visited the new manufacturing plant of Lonza.
Symposium on Engineering Stem Cells
Professor Peter Zandstra of the University of Toronto, is periodically visiting the Stem Cell Institute in 2008 to give a series of lectures, and to facilitate research and training collaborations on stem cell engineering. Abstract PDF
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HuGroup graduate Anne Kantardjieff talks about Transcriptome Analysis in Mammalian Cell Culture
HuGroup student Kartik Subramanian speaks on "Using Stem Cells to Obtain Functional Liver Hepatocytes"
HuGroup members present at Stem Cell Engineering conference, Cell Culture Engineering and ACS
HuGroup members Kartik Subramanian, Anne Kantardjieffl presented the following at the 2008 ACS annual meeting in San Diego, CA.
MAPC (an adult stem cell) Differentiation to liver cell in 3D (Kartik Subramanian)
Genome-wide regulon prediction through data mining: integrating sequence, gene function(Marlene Castro)
MAPC pluri ptency and oxygen/cell density (Yonsil Park)
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Data Mining in Biomanufacturing
Modern manufacturing plants, with their every process and control step electronically controlled or monitored, generate not only the bioproducts but also tremendous amount of data. Mining this data can potentially provide much insight to the hidden information and enhance process robustness. Such work requires cross disciplinary team work. This review, coauthored by bioengineers and computer engineer, lays out the characteristics of bioprocess data and outlines the methods that can be used to mine the data. (Charaniya, S., et al. 2009. Biotechnol. Bioeng. submitted)
Uncovering Genes with Divergent mRNA-Protein Dynamics in Streptomyces coelicolor
This collaboration employed proteomic and transcriptomic technologies to compare the dynamics of mRNA and protein at a global level in Streptomyces coelicolor, an important model organism for cellular differentiation and antibiotic biosynthesis. Through rapid growth and stationary phase the change of mRNA and protein for most genes follow the same trend. Using principal component analysis to examine the time series data of both proteome and transcriptome, it was revealed that the trends for mRNA and protein differ for about 15% of genes. It was one of the rare comparisons of transcritome and proteome dynamics at a global scale.
Genome-wide transcriptome analysis reveals that a pleiotropic antibiotic regulator, AfsS, modulates nutritional stress response in Streptomyces coelicolor A3(2)
Knock-out of a two component system gene in Streptomyces coelicolor results in drastically reduced production of Red antibiotic. The effect of knock-out on transcriptome dynamics was examined using a DNA microarray. PCA was used to identify genes which may play key roles in altering the antibiotic production characteristics.
Books on Cell Culture Engineering
Dr. Hu has recently collaborated on two books, Cell Culture Engineering and Cell Culture Technology for Pharmaceutical and Cellular Therapies, which were published in 2006.
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A Bistable Gene Switch for Antibiotic Biosynthesis: The Butyrolactone Regulon in Streptomyces coelicolor
S. Coelicolor produces a signaling molecule that is used to synchronize the synthesis of antibiotics among neighbors in the environ in an ON-OFF manner, like a switch. This article asserts that the switch behavior is accomplished by the bistability behavior, a cherished subject in classical chemical reaction engineering.
Tweaking biological switches through a better understanding of bistability
Chatterjee articulated the bistability phenomena as a switch in biological pathways, differentiaion and development. Literature examples, both experimental observation and model analysis were discussed.
Mining Transcriptome Data for Function-Trait Relationship of Hyper Productivity of Recombinant Antibody
Comparative Transcriptome Analysis to Unveil Genes Affecting Recombinant Protein Productivity in Mammalian Cells
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The People
Meet the faculty, staff and students who form the Hu Group.
Former Group Members
The Hu Group is proud of its former students, scholars and staff who set the standard of excellence we continue to strive for.
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Life in the Hu Lab
The Hu Group returns from its annual weekend retreat and workshop to Itasca State Park where they also enjoyed Minnesota's winter outdoor activities. |
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The 2009 Cellular Bioprocess Technology course has just concluded. If you are interested in attending next year's course, or have questions about the course in general, please e-mail 
